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Substrate Supply for Effective Biocatalysis
Author(s) -
Kim PeiYi,
Pollard David J.,
Woodley John M.
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060314b
Subject(s) - biocatalysis , substrate (aquarium) , microbiology and biotechnology , chemistry , biochemical engineering , substrate specificity , biology , biochemistry , enzyme , catalysis , ecology , engineering , ionic liquid
Abstract Using biocatalysis for some chemical synthesis steps has unique advantages such as achieving higher product selectivity under ambient process conditions. However, a common limitation with such systems is the inhibition or toxicity posed by the starting substrate as well as limited aqueous solubility in many cases. In this review, we discuss the supply of substrate to bioconversions. The delivery of substrate via an auxiliary, which may be water‐miscible, or a second phase such as a water‐immiscible organic solvent, adsorbing resin, or a gas, is examined through recent examples in the field. Finally, guidelines for experimental planning and process considerations are suggested to facilitate the choice of substrate delivery method and accelerate process development.