Premium
Alignment‐Based Approach for Durable Data Storage into Living Organisms
Author(s) -
Yachie Nozomu,
Sekiyama Kazuhide,
Sugahara Junichi,
Ohashi Yoshiaki,
Tomita Masaru
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060261y
Subject(s) - computer science , genome , realization (probability) , computational biology , computer data storage , dna sequencing , dna , biology , algorithm , genetics , mathematics , gene , statistics , operating system
The practical realization of DNA data storage is a major scientific goal. Here we introduce a simple, flexible, and robust data storage and retrieval method based on sequence alignment of the genomic DNA of living organisms. Duplicated data encoded by different oligonucleotide sequences was inserted redundantly into multiple loci of the Bacillus subtilis genome. Multiple alignment of the bit data sequences decoded by B. subtilis genome sequences enabled the retrieval of stable and compact data without the need for template DNA, parity checks, or error‐correcting algorithms. Combined with the computational simulation of data retrieval from mutated message DNA, a practical use of this alignment‐based method is discussed.