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Beta vulgaris L. Suspension Cultures Permeabilized with Triton X‐100 Retain Cell Viability and Betacyanines Production Ability: A Digital Image Analysis Study
Author(s) -
TrejoTapia Gabriela,
CuevasCelis Jonathan,
SalcedoMorales Guadalupe,
Luis TrejoEspino José,
ArenasOcampo Martha L.,
JiménezAparicio Antonio
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0602597
Subject(s) - viability assay , chlorella vulgaris , suspension culture , biology , cell culture , neutral red , food science , botany , cell , biochemistry , cytotoxicity , in vitro , algae , genetics
The influence of Triton X‐100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non‐destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X‐100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non‐permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non‐permeabilized cultures.