Beta  vulgaris  L. Suspension Cultures Permeabilized with Triton X‐100 Retain Cell Viability and Betacyanines Production Ability: A Digital Image Analysis Study | Zendy

TrejoTapia Gabriela | Zendy; CuevasCelis Jonathan | Zendy; SalcedoMorales Guadalupe | Zendy; Luis TrejoEspino José | Zendy; ArenasOcampo Martha L. | Zendy; JiménezAparicio Antonio | Zendy

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Beta vulgaris L. Suspension Cultures Permeabilized with Triton X‐100 Retain Cell Viability and Betacyanines Production Ability: A Digital Image Analysis Study

Author(s) -

TrejoTapia Gabriela,

CuevasCelis Jonathan,

SalcedoMorales Guadalupe,

Luis TrejoEspino José,

ArenasOcampo Martha L.,

JiménezAparicio Antonio

Publication year - 2007

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp0602597

Subject(s) - viability assay , chlorella vulgaris , suspension culture , biology , cell culture , neutral red , food science , botany , cell , biochemistry , cytotoxicity , in vitro , algae , genetics

The influence of Triton X‐100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non‐destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X‐100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non‐permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non‐permeabilized cultures.

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