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Effects of Gel Concentration, Human Fibronectin, and Cation Supplement on the Tissue‐Engineered Cartilage
Author(s) -
Kuo YungChih,
Ku INan
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060253h
Subject(s) - scaffold , porosity , genipin , chemistry , chitosan , matrix (chemical analysis) , chemical engineering , fibronectin , gelatin , tissue engineering , extracellular matrix , materials science , chromatography , biomedical engineering , biochemistry , organic chemistry , medicine , engineering
Cultivation of bovine knee chondrocytes (BKCs) in various cationic additives was studied using chitosan‐gelatin scaffolds, whose surfaces were modified by human fibronectin (HFN). Here, the genipin‐crosslinked scaffolds were fabricated by the freezing/lyophilization method with various concentrations of the precursory gels. The experimental results indicated that a lower freezing temperature led to higher moisture content, porosity, and specific surface area of a scaffold. The higher the precursor concentration, the larger the moisture content of a scaffold. A fast biodegradation of scaffold matrix was generated by a high porosity with BKCs. A higher concentration of HFN coated on scaffold surfaces yielded a faster rate of BKC attachment from the culture medium. The amounts of BKCs, glycosaminoglycans, and collagen over 28‐day cultivation increased with the scaffold porosity, the coating concentration of HFN, the seeding density of BKCs, and the calcium concentration in medium.