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High Concentration Cultivation of Bifidobacterium bifidum in a Submerged Membrane Bioreactor
Author(s) -
Kwon SounGyu,
Son JuWan,
Kim HyeJung,
Park ChangSu,
Lee JungKul,
Ji Geun Eog,
Oh DeokKun
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060236s
Subject(s) - bifidobacterium bifidum , bioreactor , ammonium , chromatography , membrane bioreactor , chemistry , hydraulic retention time , membrane , food science , dilution , biology , fermentation , biochemistry , effluent , botany , bifidobacterium , lactobacillus , environmental engineering , physics , organic chemistry , engineering , thermodynamics
In batch cultures, after 25 h, the maximum cell mass of Bifidobacterium bifidum BGN4 was 4.5 g/L, and the maximum cell count was 3.0 × 10 9 cfu/mL at pH 6.0 and 50 g/L sucrose. To increase the viable counts of bifidobacteria, cell retentive culture was applied using a submerged membrane bioreactor with suction and gas sparging. The maximum mass, count, and productivity of the cells after 36 h were 12.0 g/L, 2.2 × 10 10 cfu/mL, and 6.1 × 10 8 cfu/mL·h, respectively, at the feeding (dilution) rate of 120 mL/h (0.06 h −1 ) in the feeding medium. The accumulated levels of organic acids and ammonium ions at the end of the cultivation were 1.5 and 1.0 g/L, respectively. The viable counts and volumetric productivity of the cells after the cell retentive culture were 7.3‐ and 5.1‐fold higher, respectively, than the values obtained during batch culture. These high viable counts and volumetric productivities were obtained by maintaining lower concentrations of organic acids and ammonium ions so that the growth of B. bifidum BGN4 was not inhibited. The submerged membrane bioreactor produced the highest viable counts of B. bifidum without membrane fouling and cell damage.