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Aptamer‐Mediated Magnetic and Gold‐Coated Magnetic Nanoparticles as Detection Assay for Prion Protein Assessment
Author(s) -
Kouassi Gilles K.,
Wang Ping,
Sreevatan Srinand,
Irudayaraj Joseph
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0602101
Subject(s) - biotinylation , aptamer , magnetic nanoparticles , colloidal gold , chemistry , nanoparticle , conjugated system , biophysics , surface modification , streptavidin , transmission electron microscopy , fourier transform infrared spectroscopy , surface plasmon resonance , nanotechnology , combinatorial chemistry , biochemistry , materials science , biotin , microbiology and biotechnology , chemical engineering , polymer , organic chemistry , biology , engineering
This article reports the chemical synthesis and functionalization of magnetic and gold‐coated magnetic nanoparticles. The binding characteristics of streptavidin‐conjugated nanoparticles were studied using prion protein as a target to a specific biotinylated aptamer. The size and structure of the particles were determined by transmission electron microscopy, and the binding of the prion to the particle was confirmed by Fourier transform infrared spectroscopy. The rate of prion binding to the aptamer was dose‐dependent, and prion immobilization was more effective on l ‐aspartic acid‐functionalized magnetic nanoparticles compared to the carboxyl‐functionalized gold‐coated magnetic nanoparticles. This study sets the stage for the development of prion detection platforms as well as our long‐term goals in structure elucidation at the binding interface.