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Efficient Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus
Author(s) -
Ikeda Yuko,
Hayashi Hiroyuki,
Okuda Naoyuki,
Park Enock Y.
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060201s
Subject(s) - acremonium , cellulase , fungus , filamentous fungus , microbiology and biotechnology , biology , chemistry , enzyme , botany , biochemistry
Cellulase production was investigated in a culture of a strain of Acremonium cellulolyticus. The medium components were optimized for the improvement of cellulase production. The maximum production of cellulolytic enzymes was obtained in a medium containing (grams per liter) 50 Solka Floc, 5 (NH 4 ) 2 SO 4 , 24 KH 2 PO 4 , 4.7 potassium tartrate hemihydrate, 1.2 MgSO 4 ·7H 2 O, 1 Tween 80, 4 urea, 0.01 ZnSO 4 ·7H 2 O, 0.01 MnSO 4 ·6H 2 O, and 0.01 CuSO 4 ·7H 2 O, with a pH of 4.0. In the flask culture, 15.5 filter paper units (FPU)/mL of maximum cellulase activity was obtained, 17.42 FPU/mL in a 7‐L bioreactor, and 13.08 FPU/mL in a 50‐L scale bioreactor for 4–8 d at 30 °C. Average production rates were 1.94 FPU/mL·d in flasks, 2.86 FPU/mL·d in the 7‐L bioreactor, and 2.56 FPU/mL·d in the 50‐L bioreactor. Cellulase production on a small scale was successfully reproduced in the 50‐L pilot scale bioreactor. Saccharification activity from A. cellulolyticus was compared with cellulolytic enzymes produced by other strains. The A. cellulolyticus culture broth had a comparable saccharification yield in comparison with those of other Trichoderma enzymes (GC220 or Cellulosin T2) under the same total cellulase activity. Its saccharification yield (percent of released reducing sugar to used dried substrate) was 60%, and its glucose content was 83%.