Comparative Evaluation of Different DNA Extraction Procedures from Food Samples | Zendy

Bernardo G. Di | Zendy; Gaudio S. Del | Zendy; Galderisi U. | Zendy; Cascino A. | Zendy; Cipollaro M. | Zendy

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Comparative Evaluation of Different DNA Extraction Procedures from Food Samples

Author(s) -

Bernardo G. Di,

Gaudio S. Del,

Galderisi U.,

Cascino A.,

Cipollaro M.

Publication year - 2007

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp060182m

Subject(s) - extraction (chemistry) , dna extraction , dna , chromatography , biology , food science , microbiology and biotechnology , chemistry , genetics , polymerase chain reaction , gene

Five methodologies for extracting DNA from food samples are described. The food products analyzed are from either soybean or maize. They were selected on the basis of the mechanical, thermal, and chemical treatments that they had been subjected to during industrial processing. DNA preparations were evaluated for purity, yield, and average fragment size. Two endogenous genes, soybean lectin gene and alcohol dehydrogenase gene ( adh1 ), were used to assess the degree of DNA degradation at different stages of the transformation chain. The goal of this study was to determine the role that extraction methods play in DNA amplification in order to select the best protocol for a food sample. This comparative evaluation can be specifically useful for detection of genetically modified ingredients in a variety of food matrices.

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