Premium
Comparative Evaluation of Different DNA Extraction Procedures from Food Samples
Author(s) -
Bernardo G. Di,
Gaudio S. Del,
Galderisi U.,
Cascino A.,
Cipollaro M.
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060182m
Subject(s) - dna extraction , dna , extraction (chemistry) , alcohol dehydrogenase , transformation (genetics) , biology , microbiology and biotechnology , food science , genetically modified organism , gene , food processing , chromatography , polymerase chain reaction , chemistry , biochemistry , alcohol
Five methodologies for extracting DNA from food samples are described. The food products analyzed are from either soybean or maize. They were selected on the basis of the mechanical, thermal, and chemical treatments that they had been subjected to during industrial processing. DNA preparations were evaluated for purity, yield, and average fragment size. Two endogenous genes, soybean lectin gene and alcohol dehydrogenase gene ( adh1 ), were used to assess the degree of DNA degradation at different stages of the transformation chain. The goal of this study was to determine the role that extraction methods play in DNA amplification in order to select the best protocol for a food sample. This comparative evaluation can be specifically useful for detection of genetically modified ingredients in a variety of food matrices.