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Photoimmobilization for Microarrays
Author(s) -
Ito Yoshihiro
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060143a
Subject(s) - biochip , dna microarray , proteomics , microarray , protein microarray , antibody microarray , computational biology , chemistry , gene chip analysis , protein array analysis , microbiology and biotechnology , antibody , nanotechnology , biology , biochemistry , materials science , gene , gene expression , genetics
A photoimmobilization method has been developed for the preparation of microarray biochips. This photoimmobilization method makes it possible to easily covalently immobilize various types of organic molecules and cells on a chip. In addition, by using hydrophilic polymers as matrixes, it is possible to reduce nonspecific interactions with biological components. Various proteins, antibodies, and cells have been microarrayed using this technique, and interactions between these proteins, antibodies, and cells have been investigated. This type of microarray biochip will be important for academic applications such as genomics, proteomics, and cellomics, and clinical analyses.