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Purification of RT‐PCR Competent Poly(A) mRNA from Crude Cell Lysate by Affinity Precipitation
Author(s) -
Stocker Gisela,
Vandevyver Caroline,
Hilbrig Frank,
Freitag Ruth
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp060095u
Subject(s) - biotinylation , messenger rna , lysis , chemistry , precipitation , chromatography , coprecipitation , microbiology and biotechnology , biochemistry , biology , organic chemistry , physics , meteorology , gene
Abstract Stimuli‐responsive bioconjugates consisting of avidin covalently linked to poly( N ‐isopropylacrylamide) were used for the recovery of poly(A) mRNA hybridized to biotinylated poly(dT)‐tags from crude cell lysates (Jurkat cells) by affinity precipitation. The bioconjugates are soluble in cold water but precipitate readily once a critical solution temperature (33 °C in pure water) is surpassed. The process is fully reversible and shows the expected dependencies on the composition of the aqueous solution and the bioconjugate chemistry. The results of the affinity precipitation were compared to those achieved with an accepted standard purification of poly(A) mRNA using avidin‐activated magnetic beads. Both yield and quality/purity of the affinity precipitated poly(A) mRNA were found to be similar or better (especially removal of rRNA) than for poly(A) mRNA prepared by the magnetic particle‐based protocol, while both mRNA isolates performed equally well in standard reverse transcriptase amplification (RT‐PCR) of a β actin transcript fragment. Poly(A) mRNA purification schemes based on affinity precipitation require no dedicated equipment and should have advantages in terms of scalability, handling, and costs.