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Asymmetric Reduction of o ‐Chloroacetophenone with Candida pseudotropicalis 104
Author(s) -
Xie Qing,
Wu Jianping,
Xu Gang,
Yang Lirong
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0600583
Subject(s) - substrate (aquarium) , chemistry , cofactor , enantiomeric excess , ethanol , ketone , stereochemistry , enantiomer , biochemistry , biology , enantioselective synthesis , enzyme , organic chemistry , catalysis , ecology
The asymmetric reduction of o ‐chloroacetophenone 1 with Candida pseudotropicalis 104 produced the corresponding ( S )‐1‐(2‐chloro‐phenyl)‐ethanol 2 with the enantiomeric excess (ee >99%) without addition of any cosolvent. The cell could tolerate high ketone 1 concentration of 233.8 mmol/L (i.e., 36 g/L) with considerable reduction activity in this method. The product 2 concentration achieved 38.9 and 58.4 mmol/L with cells of 40 and 60 g DCW (dry cell weight)'L, respectively, in 24 h. The optimum reaction time, the effect of substrate concentration, cosubstrate type and concentration, and cell concentration in the reaction were investigated in this paper.