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Monitoring a Complex Medium Fermentation with Sample‐Sample Two‐Dimensional FT‐NIR Correlation Spectroscopy
Author(s) -
Ferreira Ana P.,
Menezes José C.
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0503991
Subject(s) - fermentation , two dimensional nuclear magnetic resonance spectroscopy , spectroscopy , analytical chemistry (journal) , chemistry , sample (material) , chromatography , food science , physics , stereochemistry , quantum mechanics
The possibility of monitoring state transitions during the time course of a fermentation through the analysis of the on‐line collected near‐infrared (NIR) spectra with sample‐sample two‐dimensional correlation spectroscopy (SS‐2DCoS) was investigated. SS‐2DCoS has proved to be useful for extracting process information directly from the spectra collected on‐line. The complexity of the system studied prevented the extraction of concentration profiles, but nevertheless, the application of SS‐2DCoS enables the identification of fermentation state transitions due to metabolic and morphological changes of the microorganism.