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Characteristics of a Mesoporous Silicate Immobilized Trypsin Bioreactor in Organic Media
Author(s) -
Goradia Dimple,
Cooney Jakki,
Hodnett B. Kieran,
Magner Edmond
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp050334y
Subject(s) - bioreactor , trypsin , mesoporous material , chemistry , silicate , chromatography , chemical engineering , biochemistry , organic chemistry , enzyme , catalysis , engineering
Mesoporous silicates (MPS) materials are attractive materials for immobilizing proteins/enzymes because of their well ordered structures, large surface areas (up to 1000 m 2 g −1 ), narrow pore size distributions, large pore diameters, and pore volumes. MPS with average pore diameters ranging from 28 to 300 Å were prepared using cationic and nonionic surfactants. The influence of water content, pH, storage, and thermal treatment on the activity of trypsin immobilized onto MPS was investigated. In a range of solvents, the amidolytic activity of immobilized trypsin was higher than that of the lyophilized preparation. Significant increases in k cat / K M occurred in propanol, ethanol, methanol, and formamide of 90, 62, 45 and 26, respectively. The observed increases were primarily a result of substantial increases in k cat .