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Relationship between Debaryomyces pseudopolymorphus Enzymatic Extracts and Release of Terpenes in Wine
Author(s) -
Villena María Arévalo,
Iranzo Juan Úbeda,
Briones Pérez Ana
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp050245l
Subject(s) - wine , chemistry , enzyme , terpene , liberation , enzymatic hydrolysis , food science , glycoside , hydrolysis , yeast , enzyme assay , chromatography , biochemistry , organic chemistry , in vitro
As a result of the interest that exists in the liberation of aromas in young wines, we obtained some different enzymatic extracts (purified extract, P; lyophilized purified extract, LP; immobilized purified extract, IP; and immobilized lyophilized purified extract, ILP) with β‐glucosidase activity from Debaryomyces pseudopolymorphus , which excreted the enzyme in the growth medium. The extracts were added to natural glycosides isolated from different grape varieties. The results were compared with the effect of seven commercial enzyme preparations (CEP), obtained from molds used in wine making. It was shown that some yeast extracts had effects similar to those of the CEP, and the next step was to use them on wine samples elaborated in the laboratory. The effect was studied at 9 and 16 days of contact, quantifying both the precursors that were retained and the liberated terpenes. The results were compared with a control wine (without any extract) and with wine treated with a commercial enzyme preparation specially indicated for the liberation of aromas. It was observed that the enzymatic extracts from Db. pseudopolymorphus hydrolyzed the precursors in wine and that they could compete with the commercial preparations since the liberation was produced in even less time.