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Novel Chemically Synthesized Salicylate Derivative as an Effective Elicitor for Inducing the Biosynthesis of Plant Secondary Metabolites
Author(s) -
Qian ZhiGang,
Zhao ZhenJiang,
Xu Yufang,
Qian Xuhong,
Zhong JianJiang
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0502330
Subject(s) - elicitor , salicylic acid , biosynthesis , bioassay , secondary metabolism , chemistry , plant cell , cell culture , biochemistry , metabolism , biology , enzyme , gene , genetics
Trifluoroethyl salicylate (TFESA), a novel salicylate derivative, was chemically synthesized and evaluated by bioassay as a potential elicitor for inducing the biosynthesis of plant secondary metabolites. A cell line of Taxus chinensis , which stably produced a high level of bioactive taxuyunnanine C (Tc), was taken as a model plant cell system. The application of TFESA to the cell cultures could significantly induce Tc biosynthesis, although the cell growth was slightly inhibited. More interestingly, Tc production was enhanced more in the presence of TFESA compared with a structure‐similar well‐known elicitor, salicylic acid (SA). For example, addition of 100 μM TFESA on day 7 led to a high Tc content of 21.9 ± 0.1 mg g −1 (at day 21), whereas the Tc content was 14.0 ± 0.2 and 16.7 ± 0.3 mg g −1 for the control and that with addition of 100 μM SA, respectively. The results indicate that the newly synthesized TFESA can act as a powerful elicitor for secondary metabolism induction in plant cell cultures.