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Polymer‐Templated Microarrays for Highly Reliable Plaque Purification
Author(s) -
Na Kyounga,
Lee Myungsoon,
Shin Byungcheol,
Je Yeonho,
Hyun Jinho
Publication year - 2006
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp050221e
Subject(s) - micropatterning , multiplicity of infection , green fluorescent protein , substrate (aquarium) , confocal microscopy , cell , fluorescence microscope , materials science , nanotechnology , biophysics , chemistry , microbiology and biotechnology , fluorescence , biology , biochemistry , ecology , physics , quantum mechanics , gene
To infect cells with a particular multiplicity of infection, it is essential to know the concentration of virus in the inoculum. Here we describe a highly reliable and controllable method for plaque purification using cell‐repellent surfaces micropatterned on the substrate. Micropatterning of localized chemical or biochemical domains has the potential to become a powerful tool in controlling the seeding of cells. The cell array was reliably fabricated with micropatterned surfaces, and the number of cells in a pattern was easily controlled by the cell density in the media and micropattern size. The cell micropatterns were infected with baculoviruses to form an array of virus plaques. GFP‐modified and wild‐type baculoviruses were used to verify the feasibility of purifying a specific plaque. Using confocal microscopy, GFP‐expressing plaques were readily selectable and removable.