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Production of Oligoglucuronans by Enzymatic Depolymerization of Nascent Glucuronan
Author(s) -
Delattre Cédric,
Michaud Philippe,
Keller Cyril,
Courtois Bernard,
Courtois Josiane
Publication year - 2005
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0501930
Subject(s) - depolymerization , bioreactor , chemistry , ultrafiltration (renal) , acetylation , biosynthesis , enzyme , chromatography , polymerization , biochemistry , organic chemistry , polymer , gene
An original bioreactor process for production of oligoglucuronans was developed using the Sinorhizobium meliloti M5N1CS strain that produces glucuronan. This anionic homopolysaccharide was composed of β ‐ d ‐(1,4)‐glucopyranosyluronic residues variably O ‐acetylated at C‐3 and/or C‐2 positions according to culture conditions. It was depolymerized during its biosynthesis by addition of a fungal glucuronan lyase activity in broths. After purification by tangential ultrafiltration and low‐pressure liquid chromatography, 1 H NMR and ESI‐Q/TOF‐MS characterized the poly‐ and oligoglucuronic acid fractions. This enzymatic bioreactor strategy authorized the production in gram quantity of an unsaturated and no acetylated oligoglucuronan with a degree of polymerization of 3.