Production of Oligoglucuronans by Enzymatic Depolymerization of Nascent Glucuronan | Zendy

Delattre Cédric | Zendy; Michaud Philippe | Zendy; Keller Cyril | Zendy; Courtois Bernard | Zendy; Courtois Josiane | Zendy

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Production of Oligoglucuronans by Enzymatic Depolymerization of Nascent Glucuronan

Author(s) -

Delattre Cédric,

Michaud Philippe,

Keller Cyril,

Courtois Bernard,

Courtois Josiane

Publication year - 2005

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp0501930

Subject(s) - depolymerization , bioreactor , chemistry , ultrafiltration (renal) , acetylation , biosynthesis , enzyme , chromatography , polymerization , biochemistry , organic chemistry , polymer , gene

An original bioreactor process for production of oligoglucuronans was developed using the Sinorhizobium meliloti M5N1CS strain that produces glucuronan. This anionic homopolysaccharide was composed of β ‐ d ‐(1,4)‐glucopyranosyluronic residues variably O ‐acetylated at C‐3 and/or C‐2 positions according to culture conditions. It was depolymerized during its biosynthesis by addition of a fungal glucuronan lyase activity in broths. After purification by tangential ultrafiltration and low‐pressure liquid chromatography, 1 H NMR and ESI‐Q/TOF‐MS characterized the poly‐ and oligoglucuronic acid fractions. This enzymatic bioreactor strategy authorized the production in gram quantity of an unsaturated and no acetylated oligoglucuronan with a degree of polymerization of 3.

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