Premium
A Note Concerning Acetate Activation of Peroxidative Activity of Catalases Using 2,2′‐Azino‐bis(3‐ethylbenzthiazoline)‐6‐sulfonic Acid as a Substrate
Author(s) -
Baker Warren L.,
Key Christopher,
Lonergan Greg T.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0500617
Subject(s) - chemistry , hydrogen peroxide , substrate (aquarium) , catalysis , abts , propionate , catalase , sulfonic acid , organic chemistry , nuclear chemistry , medicinal chemistry , enzyme , antioxidant , oceanography , dpph , geology
Beef liver catalases showed peroxidative activity using 2,2′‐azino‐bis‐(3‐ethylbenzthiazoline)‐6‐sulfonic acid as the electron donor and hydrogen peroxide as the acceptor at a pH of 5. This activity was not observed at pH 7. The reaction depended on acetate concentration, although succinate and propionate could partly replace the acetate as a catalyst. Other haem proteins also catalyzed a peroxidative effect. The reaction using syringaldazine or the coupling between dimethylaminobenzoic acid and 3‐methyl‐2‐benzothiazolinone hydrazone was less effective and less sensitive. Evidence is presented that the reaction is associated with a conformational change of the catalase.