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Contact Angle, WAXS, and SAXS Analysis of Poly(β‐hydroxybutyrate) and Poly(ethylene glycol) Block Copolymers Obtained via Azotobacter vinelandii UWD
Author(s) -
Townsend Kerry J.,
Busse Karsten,
Kressler Joerg,
Scholz Carmen
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp050044h
Subject(s) - azotobacter vinelandii , small angle x ray scattering , copolymer , ethylene glycol , polymer chemistry , materials science , azotobacteraceae , chemistry , polymer , organic chemistry , scattering , composite material , optics , physics , nitrogen fixation , nitrogenase , nitrogen
This study investigated and correlated physical properties and cell interactions of copolymers obtained by a poly(ethylene glycol) (PEG)‐modulated fermentation of Azotobacter vinelandii UWD. PEGs with molecular weights of 400 and 3400 Da and di(ethylene glycol) (DEG) were used to modulate the bacterial synthesis of poly(β‐hydroxybutyrate) (PHB). The PHB crystallinity was determined by wide‐angle X‐ray scattering (WAXS). Small‐angle X‐ray scattering (SAXS) showed that lamellar distances decreased between the PHB and the PHB modulated with PEG or DEG. Furthermore, the contact angle of water on the PHB/PEG polymer surfaces decreased when compared to that of PHB. The significant decrease of the contact angle and corresponding increase in surface tension, as well as significant decrease in cell adhesion, suggest the presence of hydrophilic PEG and DEG within the hydrophobic surface.