Premium
Quorum‐Sensing‐Based Toolbox for Regulatable Transgene and siRNA Expression in Mammalian Cells
Author(s) -
Weber Wilfried,
Malphettes Laetitia,
Rinderknecht Matthias,
Schoenmakers Ronald G.,
Spielmann Manuela,
Keller Bettina,
Van de Wetering Petra,
Weber Cornelia C.,
Fussenegger Martin
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0498995
Subject(s) - transgene , biology , gene expression , rna interference , gene , transduction (biophysics) , computational biology , transcription (linguistics) , streptomyces coelicolor , microbiology and biotechnology , genetics , rna , mutant , biochemistry , linguistics , philosophy
Technologies for regulated expression of multiple transgenes in mammalian cells have gathered momentum for bioengineering, gene therapy, drug discovery, and gene‐function analyses. Capitalizing on recently developed mammalian transgene modalities (QuoRex) derived from Streptomyces coelicolor , we have designed a flexible and highly compatible expression vector set that enables desired transgene/siRNA control in response to the nontoxic butyrolactone SCB1. The construction‐kit‐like expression portfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional (pBiRex), (iv) oncoretro‐ and lentiviral transduction, and (v) RNA polymerase II‐based siRNA transcription‐fine‐tuning vectors for straightforward implementation of QuoRex‐controlled (trans)gene modulation in mammalian cells.