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Bacterial Cellulose Production by Fed‐Batch Fermentation in Molasses Medium
Author(s) -
Bae Sangok,
Shoda Makoto
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0498490
Subject(s) - fermentation , food science , sugar , bacterial cellulose , chemistry , cellulose , reducing sugar , batch reactor , carbon source , biochemistry , catalysis
Batch and fed‐batch fermentations for bacterial cellulose (BC) production using molasses as a carbon source by Acetobacter xylinum BPR2001 were carried out in a jar fermentor. For improvement of BC production, molasses was subjected to H 2 SO 4 –heat treatment. The maximum BC concentration by this treated molasses increased 76%, and the specific growth rate increased 2‐fold compared with that by untreated molasses. In batch fermentation, when the initial sugar concentrations of H 2 SO 4 –heat‐treated molasses were varied from 20 to 70 g/L, the highest value of maximum BC concentration of 5.3 g/L was observed at 20 g/L. BC production in intermittent fed‐batch (IFB) fermentation was conducted referring to the data in batch fermentation, and the highest BC production of 7.82 g/L was obtained when 0.2 L of molasses medium was added five times. When continuous fed‐batch (CFB) fermentations were conducted, maximum BC concentration was obtained with a feeding rate of 6.3 g‐sugar/h, which was derived from the optimal IFB experiment.