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Effect of Production Method and Gene Amplification on the Glycosylation Pattern of a Secreted Reporter Protein in CHO Cells
Author(s) -
Lipscomb Matthew L.,
Palomares Laura A.,
Hernández Vanessa,
Ramírez Octavio T.,
Kompala Dhinakar S.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp049761m
Subject(s) - chinese hamster ovary cell , glycosylation , cell culture , biology , microbiology and biotechnology , recombinant dna , reporter gene , n linked glycosylation , gene , hamster , transfection , biochemistry , bioreactor , glycan , glycoprotein , gene expression , genetics , botany
We have investigated the independent effects of selective gene amplification (using the dhfr amplifiable selection marker) and culture operating strategy (batch vs repeated fed‐batch vs semicontinuous perfusion) on the glycosylation of a recombinant reporter protein (secreted alkaline phosphatase, SEAP) produced in transfected Chinese hamster ovary (CHO) cells. HPLC analyses coupled with susceptibility to various exoglycosidases were used to determine the N‐glycosylation profile of SEAP samples. The dhfr amplified cell line yielded an almost 10‐fold increase in specific productivity as compared to that of the unamplified cell line. The glycosylation pattern of the reporter protein produced in batch bioreactor cultures of the amplified cell line showed only slight differences as compared to the glycosylation pattern of the protein from batch bioreactor cultures of the unamplified cell line. In contrast, analysis of SEAP glycosylation structures from the protein isolated from semicontinuous perfusion cultures indicated that both relative glycan content and extent of sialylation were increased as compared to samples isolated from repeated fed‐batch cultures. These results suggest that the slow growing perfusion cultures produce more completely glycosylated proteins than the faster growing repeated fed‐batch cultures.