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Methods To Improve the Yield and Quality of DNA from Dried and Processed Figs
Author(s) -
Bernardo G. Di,
Galderisi U.,
Cipollaro M.,
Cascino A.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp049710p
Subject(s) - dna , yield (engineering) , genome , dna extraction , computational biology , biology , microbiology and biotechnology , polymerase chain reaction , chemistry , genetics , gene , materials science , metallurgy
We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.