Optimization of Rosmarinic Acid Production by Lavandula vera MM Plant Cell Suspension in a Laboratory Bioreactor

The all‐round effect of dissolved oxygen concentration, agitation speed, and temperature on the rosmarinic acid production by Lavandula vera MM cell suspension was studied in a 3‐L laboratory bioreactor by means of the modified Simplex method. Polynomial regression models were elaborated for description of the process of rosmarinic acid production ( Y ) in the bioreactor as a consequence of the variation of the dissolved oxygen ( X 1 ) concentration between 10% and 50%; agitation ( X 2 ) between 100 and 400 rpm; and temperature ( X 3 ) between 22 and 30 °C. The optimization made it possible to establish the optimal conditions for the biosynthesis of rosmarinic acid by L. vera MM: dissolved oxygen ( X 1 * ), 50% of air saturation; agitation ( X 2 * ), 400 rpm; and temperature ( X 3 * ), 29.9 °C, where maximal yield ( Y max ) of 3489.4 mg/L of rosmarinic acid was achieved (2 times higher compared with the shake‐flasks cultivation).