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Optimization of Rosmarinic Acid Production by Lavandula vera MM Plant Cell Suspension in a Laboratory Bioreactor
Author(s) -
Pavlov Atanas I.,
Georgiev Milen I.,
Panchev Ivan N.,
Ilieva Mladenka P.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp049678z
Subject(s) - rosmarinic acid , lavandula , bioreactor , suspension culture , suspension (topology) , chemistry , chromatography , biology , botany , lavender , cell culture , biochemistry , mathematics , genetics , homotopy , essential oil , pure mathematics , antioxidant
The all‐round effect of dissolved oxygen concentration, agitation speed, and temperature on the rosmarinic acid production by Lavandula vera MM cell suspension was studied in a 3‐L laboratory bioreactor by means of the modified Simplex method. Polynomial regression models were elaborated for description of the process of rosmarinic acid production ( Y ) in the bioreactor as a consequence of the variation of the dissolved oxygen ( X 1 ) concentration between 10% and 50%; agitation ( X 2 ) between 100 and 400 rpm; and temperature ( X 3 ) between 22 and 30 °C. The optimization made it possible to establish the optimal conditions for the biosynthesis of rosmarinic acid by L. vera MM: dissolved oxygen ( X 1 * ), 50% of air saturation; agitation ( X 2 * ), 400 rpm; and temperature ( X 3 * ), 29.9 °C, where maximal yield ( Y max ) of 3489.4 mg/L of rosmarinic acid was achieved (2 times higher compared with the shake‐flasks cultivation).