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Production of Human α‐1‐Antitrypsin from Transgenic Rice Cell Culture in a Membrane Bioreactor
Author(s) -
McDonald Karen A.,
Hong Lo Ming,
Trombly David M.,
Xie Qing,
Jackman Alan P.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0496676
Subject(s) - bioreactor , compartment (ship) , extracellular , cell culture , genetically modified rice , transgene , recombinant dna , chemistry , cell , biology , biochemistry , microbiology and biotechnology , genetically modified crops , gene , botany , oceanography , genetics , geology
Transgenic plant cell cultures offer a number of advantages over alternative host expression systems, but so far relatively low product concentrations have been achieved. In this study, transgenic rice cells are used in a two‐compartment membrane bioreactor (CELLine 350, Integra Biosciences) for the production of recombinant α‐1‐antitrypsin (rAAT). Expression of rAAT is controlled by the rice α‐amylase (RAmy3D) promoter, which is induced in the absence of sugar. The extracellular product is retained in the bioreactorapos;s relatively small cell compartment, thereby increasing product concentration. Due to the packed nature of the cell aggregates in the cell compartment, a clarified product solution can be withdrawn from the bioreactor. Active rAAT reached levels of 100–247 mg/L (4–10% of the total extracellular protein) in the cell compartment at 5–6 days postinduction, and multiple inductions of the RAmy3D promoter were demonstrated.