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Retroviral Transduction of Adherent Cells in Resonant Acoustic Fields
Author(s) -
Lee YuHsiang,
You JinOh,
Peng ChingAn
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0496427
Subject(s) - transduction (biophysics) , retrovirus , 3t3 cells , fibroblast , cell culture , gene transfer , gene delivery , genetic enhancement , gene , biology , microbiology and biotechnology , transfection , virus , virology , biophysics , genetics
Ultrasound‐induced cavitation has been extensively used to enhance the efficiency of nonviral‐based gene delivery. Although such unique mechanical force could possibly augment the efficacy of retrovirus‐mediated gene transfer, we harnessed an alternative approach, a resonant acoustic field, to facilitate the retroviral transduction rate. NIH 3T3 fibroblast cells suspended in a culture well and mixed with ecotropic retroviruses were co‐treated with megahertz resonant acoustic fields (RAF). Suspended NIH 3T3 cells under RAF treatment agglomerated at acoustic nodal planes by primary radiation force within a short exposure time. These first arrived and agglomerated cells formed bands as nucleating sites for nanometer‐sized ecotropic retroviruses circulated between nodal planes to attach on and thereby increased cell‐virus encounters. According to the neomycin‐resistant colony assay, 2‐fold increment of retroviral transduction rate was obtained by exposing cells and retroviruses in the RAF for 6 min in the presence of 8 μg/mL Polybrene.