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Effect of Medium Composition on the Production of Tetanus Toxin by Clostridium tetani
Author(s) -
Fratelli Fernando,
Siquini Tatiana Joly,
Prado Sally Müller Affonso,
Higashi Hisako Gondo,
Converti Attilio,
de Carvalho João Carlos Monteiro
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp049571b
Subject(s) - clostridium tetani , toxoid , toxin , tetanus , neurotoxin , microbiology and biotechnology , chemistry , biology , food science , biochemistry , virology , vaccination
The tetanus toxin is a neurotoxin synthesized by the bacillus Clostridium tetani that, after detoxification with formaldehyde, still exhibits antigenic and immunologic properties, hence its denomination of tetanus toxoid. Such a neurotoxin is produced by cultivation of the microorganism in vegetative form on a relatively complex specific medium containing glucose and peptone. The simultaneous effects of the starting levels of glucose ( G 0 ) and N‐Z Case TT ( NZ 0 ) as carbon and nitrogen sources, respectively, on the production of tetanus toxin have been investigated in this work in static cultivations by means of a five‐level star‐shaped experimental design and evaluated by response surface methodology (RSM) for optimization purposes. The highest final average yield of tetanus toxin (72 L f /mL), achieved at G 0 = 9.7 g/L and NZ 0 = 43.5 g/L, was 80% higher than that obtained with standard cultivations ( G 0 = 8.0 g/L and NZ 0 = 25.0 g/L).