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Kinetic Study of Cell Proliferation of Saccharomyces cerevisiae Strains by Sedimentation/Steric Field Flow Fractionation in Situ
Author(s) -
Farmakis Lambros,
Koliadima Athanasia
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0495466
Subject(s) - yeast , saccharomyces cerevisiae , fractionation , field flow fractionation , sedimentation , cell fractionation , budding yeast , fission , in situ , budding , steric effects , chemistry , biology , chromatography , scanning electron microscope , microbiology and biotechnology , biochemistry , materials science , enzyme , stereochemistry , paleontology , sediment , neutron , composite material , physics , organic chemistry , quantum mechanics
The technique of Sedimentation/Steric Field Flow Fractionation (Sd/StFFF) is applied to the kinetic study of cells proliferation of Saccharomyces cerevisiae strains. The experimental parameter varied is the time from the preparation of the yeast sample dispersion in the culture medium. The determination of the size and mass distributions of the yeast cells is combined with the growth of the yeast cells and their life cycle. The experimental results are compared with those obtained by scanning electron microscopy (SEM) and those found in the literature. Useful conclusions concerning the budding and the fission of these yeast cells were extracted.