Premium
Pseudomonas fluorescens 134 as a Biological Control Agent (BCA) Model in Cell Immobilization Technology
Author(s) -
Russo Anna,
Basaglia Marina,
Casella Sergio,
Nuti Marco Paolo
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp040030w
Subject(s) - pseudomonas fluorescens , rhizoctonia solani , inoculation , chemistry , pseudomonas , population , dissolution , biological pest control , strain (injury) , sodium alginate , pseudomonadales , microbiology and biotechnology , food science , horticulture , biology , sodium , bacteria , genetics , demography , organic chemistry , anatomy , sociology
Antifungal activity against Rhizoctonia solani was achieved in vivo through the application of Pseudomonas fluorescens strain 134 encapsulated in sodium alginate beads of different sizes (0.5, 1, and 2 mm). The activity was compared to that obtainable with chemical treatments and bead‐derived liquid formulations. The latter was obtained by dissolving alginate beads of 1 and 0.5 mm in 1% Na‐citrate solution before application, without any significant ( P < 0.05) reduction of bacterial numbers during the dissolution process. The dry bead formulations were applied next to the seeds in plant inoculation experiments, resulting in a reduction of disease symptoms, which were markedly reduced when the liquid formulation was applied. Moreover, the rate of disease symptoms related to liquid formulations from both 1 and 0.5 mm beads was comparable (near to 10%) to that of chemical treatment. Pseudomonas fluorescens strain 134 delivered as both dry and liquid formulations was able to colonize cotton root at a population density of about 10 8 CFU/g fresh root, 15 days after sowing.