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Evaluation of Microbeads of Calcium Alginate as a Fluidized Bed Medium for Affinity Chromatography of Aspergillus n iger Pectinase
Author(s) -
Roy Ipsita,
Jain Sulakshana,
Teotia Sunita,
Gupta Munishwar Nath
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0343864
Subject(s) - chromatography , fluidized bed , pectinase , aspergillus niger , calcium alginate , fluidization , chemistry , calcium , dispersion (optics) , biochemistry , enzyme , organic chemistry , physics , optics
Calcium alginate microbeads (212–425 μm) were prepared by spraying 2% (w/v) alginate solution into 1 M CaCl 2 solution. The fluidization behavior of these beads was studied, and the bed expansion index and terminal velocity were found to be 4.3 and 1808 cm h −1 , respectively. Residence time distribution curves showed that the dispersion of the protein was much less with these microbeads than with conventionally prepared calcium alginate macrobeads when both kinds of beads were used for chromatography in a fluidized bed format. The fluidized bed of these beads was used for the purification of pectinase from a commercial preparation. The media performed well even with diluted feedstock; 90% activity recovery with 211‐fold purification was observed.