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Solid‐State Fermentation for Production of Griseofulvin on Rice Bran Using Penicillium griseofulvum
Author(s) -
Saykhedkar Sayali S.,
Singhal Rekha S.
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0343662
Subject(s) - griseofulvin , bran , fermentation , solid state fermentation , food science , chemistry , biomass (ecology) , penicillium , biology , agronomy , raw material , organic chemistry , medicine , pathology
Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid‐state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis‐à‐vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10 6 spores/mL under agitation at 250 rpm containing the modified Czapek‐Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (μg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.