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Reduction of Active Elastase Concentration by Means of Immobilized Inhibitors: A Novel Therapeutic Approach
Author(s) -
Grano Valentina,
Tasco Gianluca,
Casadio Rita,
Diano Nadia,
Portaccio Marianna,
Rossi Sergio,
Bencivenga Umberto,
Compiani Mario,
de Maio Anna,
Mita Damiano Gustavo
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp034304b
Subject(s) - glutaraldehyde , chemistry , membrane , covalent bond , trypsin , extracorporeal circulation , chromatography , enzyme , combinatorial chemistry , biochemistry , organic chemistry , medicine , surgery
The inhibitory power of three different active Nylon membranes, separately loaded with three different protease inhibitors, was studied with the aim of reducing the increased elastase concentration occurring during hemodialysis or extracorporeal blood circulation in patients undergoing cardiopulmonary bypass. Chemical grafting was carried out to make the inert Nylon membrane suitable for the immobilization of the inhibitors. The behavior of immobilized α 1 ‐antitrypsin, bovine pancreatic trypsin inhibitor (BPTI), or elastatinal was separately studied. α 1 ‐Antitrypsin and BPTI were covalently immobilized by means of a diazotization process, whereas elastatinal was covalently attached via a condensation process mediated by glutaraldehyde. The inhibitory power of each membrane type was studied as a function of the amount of immobilized inhibitor and temperature. All active membranes have shown good inhibitory power. The most efficient membrane was that loaded with α 1 ‐antitrypsin, the less efficient that with BPTI.