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Toward a Robust Model of Packing and Scale‐Up for Chromatographic Beds. 2. Flow Packing
Author(s) -
Keener Ronald N.,
Maneval James E.,
Fernandez Erik J.
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0342809
Subject(s) - rigidity (electromagnetism) , materials science , consolidation (business) , modulus , compression test , flow (mathematics) , elasticity (physics) , composite material , compression (physics) , chromatography , mathematics , chemistry , geometry , accounting , business
We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical compression (consolidation) test is used, the model captures the primary phenomena of the scale‐up process. However, moduli determined from flow‐packing experiments improve the accuracy of the predictions and show that the apparent rigidity of chromatographic resins is lower for flow packing than for mechanical compression. Using a modulus from flow‐packing experiments provided quantitative scale‐up predictions of flow packing carried out in columns with diameters between 200 and 450 mm at different locations and by different operators.