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Population Balance Approach to Modeling Hairy Root Growth
Author(s) -
Han Binbing,
Linden James C.,
Gujarathi Ninad P.,
Wickramasinghe S. Ranil
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0342304
Subject(s) - biomass (ecology) , division (mathematics) , growth model , population , root (linguistics) , biological system , elongation , balance (ability) , biology , material balance , cell division , population model , mathematics , ecology , cell , genetics , engineering , demography , materials science , arithmetic , linguistics , philosophy , mathematical economics , neuroscience , sociology , ultimate tensile strength , metallurgy , process engineering
Though numerous models have been developed to describe the growth of microbial cell cultures, far fewer models are available to describe the growth of hairy root cultures. Here a population balance model is proposed to simulate the growth of hairy roots. The model accounts for the increase in biomass due to elongation of a branch by cell division as well as the formation of new branches. The model incorporates the fact that although the likelihood of the formation of a new lateral branch is a maximum at a specific age of the parent branch, lateral branches can form over a distribution of ages of the parent branch. Model parameters are estimated using the genetic algorithm based on experimental data for batch and continuous bioreactors. The model proposed here may provide a better understanding of the increase in biomass of hairy root cultures.