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Hollow‐Fiber Enzyme Reactor Operating under Nonisothermal Conditions
Author(s) -
Diano Nadia,
Grano Valentina,
Rossi Sergio,
Bencivenga Umberto,
Portaccio Marianna,
Amato Umberto,
Carfora Francesca,
Lepore Maria,
Gaeta Francesco Saverio,
Mita Damiano Gustavo
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp034197l
Subject(s) - hexamethylenediamine , isothermal process , bioreactor , fiber , polypropylene , temperature gradient , materials science , glutaraldehyde , immobilized enzyme , substrate (aquarium) , chemistry , thermodynamics , chemical engineering , chromatography , composite material , enzyme , biochemistry , organic chemistry , physics , polyamide , oceanography , quantum mechanics , engineering , geology
A hollow‐fiber enzyme reactor, operating under isothermal and nonisothermal conditions, was built employing a polypropylene hollow fiber onto which β‐galactosidase was immobilized. Hexamethylenediamine and glutaraldehyde were used as spacer and coupling agent, respectively. Glucose production was studied as a function of temperature, substrate concentration, and size of the transmembrane temperature gradient. The actual average temperature differences across the polypropylene fiber, to which reference was done to evaluate the effect of the nonisothermal conditions, were calculated by means of a mathematical approach, which made it possible to know, using computer simulation, the radial and axial temperature profiles inside the bioreactor and across the membrane. Percent activity increases, proportional to the size of the temperature gradients, were found when the enzyme activities under nonisothermal conditions were compared to those measured under comparable isothermal conditions. Percent reductions of the production times, proportional to the applied temperature gradients, were also calculated. The advantage of employing nonisothermal bioreactors in biotechnological industrial process was discussed.