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Optimization of Cyclodextrin Glycosyltransferase Production from Klebsiella pneumoniae AS‐22 in Batch, Fed‐Batch, and Continuous Cultures
Author(s) -
Gawande Bharat N.,
Sonawane Avinash M.,
Jogdand Vithal V.,
Patkar Anant Y.
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp034115t
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , cyclodextrin , enterobacteriaceae , glycosyltransferase , klebsiella , chemistry , food science , fed batch culture , biology , escherichia coli , fermentation , biochemistry , enzyme , gene
Production of a novel cyclodextrin glycosyltransferase (CGTase) from Klebsiella pneumoniae AS‐22 strain, which converts starch predominantly to α‐CD at high conversion yields, in batch, fed‐batch, and continuous cultures, is presented. In batch fermentations, optimization of different operating parameters such as temperature, pH, agitation speed, and carbon‐source concentration resulted in more than 6‐fold increase in CGTase activity. The enzyme production was further improved by two fed‐batch approaches. First, using glucose‐based feed to increase cell density, followed by starch‐based feed to induce enzyme production, resulted in high cell density of 76 g dry cell weight/L, although the CGTase production was low. Using the second approach of a single dextrin‐based feed, 20‐fold higher CGTase was produced compared to that in batch fermentations with media containing tapioca starch. In continuous operation, more than 8‐fold increase in volumetric CGTase productivity was obtained using dextrin‐based media compared to that in batch culture using starch‐based media.