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Continuous d ‐Tagatose Production by Immobilized Thermostable l ‐Arabinose Isomerase in a Packed‐Bed Bioreactor
Author(s) -
Ryu SeAh,
Kim Chang Sup,
Kim HyeJung,
Baek Dae Heoun,
Oh DeokKun
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0340739
Subject(s) - bioreactor , packed bed , chemistry , chromatography , yield (engineering) , substrate (aquarium) , arabinose , dilution , immobilized enzyme , enzyme , fermentation , food science , biochemistry , materials science , xylose , biology , organic chemistry , ecology , physics , metallurgy , thermodynamics
d ‐Tagatose was continuously produced using thermostable l ‐arabinose isomerase immobilized in alginate with d ‐galactose solution in a packed‐bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme amount, and substrate concentration were found to be optimal at 0.8 mm, 520/7 mm, 0.375 h ‐ 1 , 5.65 units, and 300 g/L, respectively. Under these conditions, the bioreactor produced about 145 g/L tagatose with an average productivity of 54 g tagatose/L·h and an average conversion yield of 48% (w/w). Operational stability of the immobilized enzyme was demonstrated, with a tagatose production half‐life of 24 days.