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Cell Surface Display of Organophosphorus Hydrolase in Pseudomonas putida Using an Ice‐Nucleation Protein Anchor
Author(s) -
Shimazu Mark,
Nguyen Anne,
Mulchandani Ashok,
Chen Wilfred
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0340640
Subject(s) - pseudomonas putida , escherichia coli , chemistry , fusion protein , hydrolase , pseudomonas , cell , ice nucleus , enzyme , nucleation , biochemistry , biophysics , bacteria , biology , recombinant dna , organic chemistry , genetics , gene
A surface anchor system derived from the ice‐nucleation protein (INP) from Pseudomonas syringe was used to localize organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida KT2440. Cells harboring the shuttle vector pPNCO33 coding for the INP‐OPH fusion were capable of targeting OPH onto the cell surface as demonstrated by whole cell ELISA. The whole cell activity of P. putida KT2440 was shown to be 10 times higher than those of previous efforts expressing the same fusion protein in Escherichia coli . The capability of expressing enzymes on the surface of a robust and environmentally benign P. putida KT2440 should open up new avenues for a wide range of applications such as in situ bioremediation.