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Role of the Non‐Mevalonate Pathway in Indole Alkaloid Production by Catharanthus roseus Hairy Roots
Author(s) -
Hong SeungBeom,
Hughes Erik H.,
Shanks Jacqueline V.,
San KaYiu,
Gibson Susan I.
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp034031k
Subject(s) - loganin , mevalonate pathway , catharanthus roseus , alkaloid , indole alkaloid , terpenoid , biosynthesis , chemistry , botany , biochemistry , biology , enzyme , organic chemistry , high performance liquid chromatography
The 1‐deoxy‐ d ‐xylulose‐5‐phosphate (DXP) pathway (non‐mevalonate pathway) leading to terpenoids via isopentenyl diphosphate (IPP) has been shown to occur in most bacteria and in all higher plants. Treatment with the antibiotic fosmidomycin, a specific inhibitor of DXP reductoisomerase, considerably inhibited the accumulation of the alkaloids ajmalicine, tabersonine, and lochnericine by Catharanthus roseus hairy root cultures in the exponential growth phase. However, fosmidomycin did not significantly affect alkaloid levels in stationary phase hairy root cultures. Feeding with 1‐deoxy‐ d ‐xylulose, 10‐hydroxygeraniol, or loganin resulted in significant increases in alkaloid production by exponential phase hairy root cultures. These results suggest that the DXP pathway is a major provider of carbon for the monoterpenoid pathway leading to the formation of indole alkaloids in C. roseus hairy roots in the exponential phase.