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Supercritical Fluid Disruption of Ralstonia eutropha for Poly(β‐hydroxybutyrate) Recovery
Author(s) -
Hejazi Parisa,
VasheghaniFarahani Ebrahim,
Yamini Yadollah
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp034010q
Subject(s) - ralstonia , supercritical fluid , methanol , cupriavidus necator , volume (thermodynamics) , chemistry , taguchi methods , cell disruption , chromatography , bacteria , chemical engineering , materials science , polyhydroxyalkanoates , organic chemistry , biochemistry , biology , thermodynamics , composite material , gene , genetics , physics , engineering
A new method for disruption of Gram‐negative bacterium Ralstonia eutropha by supercritical CO 2 for poly(β‐hydroxybutyrate) (PHB) recovery is proposed. The effects of different parameters such as exposure time, pressure, temperature, volume of methanol as a modifier, and culture history on cell disruption efficiency were investigated using Taguchiapos;s statistical approach to determine optimum conditions. The optimum conditions for cell disruption and PHB recovery were as follows: exposure time, 100 min; pressure, 200 atm; temperature, 40 °C; volume of methanol, 0.2 mL. The cell culture time was less significant. At optimum conditions, the maximum efficiency of PHB recovery was found to be 89%. The proposed method is comparable with other recovery methods in terms of the percentage of PHB recovery, while it is environmentally more benign.