Fusion to a Carrier Protein and a Synthetic Propeptide Enhances E7 HPV‐16 Production and Secretion in  Lactococcus lactis | Zendy

BermúdezHumarán Luis G. | Zendy; CortesPerez Naima G. | Zendy; Loir Yves Le | Zendy; Gruss Alexandra | Zendy; RodriguezPadilla Cristina | Zendy; SaucedoCardenas Odila | Zendy; Langella Philippe | Zendy; Montes de OcaLuna Roberto | Zendy

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Fusion to a Carrier Protein and a Synthetic Propeptide Enhances E7 HPV‐16 Production and Secretion in Lactococcus lactis

Author(s) -

BermúdezHumarán Luis G.,

CortesPerez Naima G.,

Loir Yves Le,

Gruss Alexandra,

RodriguezPadilla Cristina,

SaucedoCardenas Odila,

Langella Philippe,

Montes de OcaLuna Roberto

Publication year - 2003

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp0340077

Subject(s) - lactococcus lactis , secretion , protein precursor , proteolysis , chemistry , protein biosynthesis , secretory protein , fusion protein , intracellular , nuclease , bacteria , biochemistry , biology , microbiology and biotechnology , recombinant dna , enzyme , gene , lactic acid , genetics

An inducible system to improve and stabilize the production of an extremely labile protein (E7 antigen of human papillomavirus type 16) was developed in the food‐grade bacterium Lactococcus lactis. A protein carrier, the staphylococcal nuclease Nuc, was fused either to N/ or C‐termini of E7 protein, and the resulting hybrid proteins were rescued from intracellular proteolysis but poorly secreted by L. lactis. A synthetic propeptide (LEISSTCDA) was then fused and significantly improved the secretion efficiency of the hybrid protein Nuc‐E7 by L. lactis .

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