Premium
Quantitative Analysis of Indigo and Indigo Precursors in Leaves of Isatis spp. and Polygonum tinctorium
Author(s) -
Gilbert Kerry G.,
Maule Hamish G.,
Rudolph Bernd,
Lewis Mervyn,
Vandenburg Harold,
Sales Ester,
Tozzi Sabrina,
Cooke David T.
Publication year - 2004
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0300624
Subject(s) - indigo , chromatography detector , botany , chemistry , high performance liquid chromatography , indigo carmine , polygonaceae , chromatography , polygonum , biology , organic chemistry , physics , optics , ecology
Analysis of extracts from two woad species ( Isatis tinctoria and Isatis indigotica ) and Polygonum tinctorium revealed that only one indigo precursor (indican) was present in Polygonum , but two precursors were found in Isatis spp. This was done using high performance liquid chromatography (HPLC), coupled to an evaporative light scattering detector (ELSD). In Isatis spp., the indigo precursors indican and a fraction representing isatan B were identified. The proportion of indican and isatan B was different between the two Isatis spp. tested. For the first time, it was possible to quantify the precursors in woad plant species, and the results were found to be in good agreement with those made from total indigo quantification using two different spectrophotometric methods or a derivatization technique.