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Auxiliary Phase Guidelines for Microbial Biotransformations of Toxic Substrate into Toxic Product
Author(s) -
Straathof A. J. J.
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp025750m
Subject(s) - biotransformation , substrate (aquarium) , chemistry , product inhibition , aqueous solution , solubility , aqueous two phase system , adsorption , phase (matter) , product distribution , process (computing) , chromatography , combinatorial chemistry , organic chemistry , chemical engineering , catalysis , enzyme , biology , ecology , non competitive inhibition , operating system , computer science , engineering
When an industrial process is developed using the microbial transformation of a precursor into a desired chemical compound, high concentrations of substrate and product will be involved. These compounds may become toxic to the cells. In situ product removal (ISPR) may be carried out, using auxiliary phases such as extractants or adsorbents. Simultaneously, in situ substrate addition (ISSA) may be performed. It is shown that for uncharged substrates and products, the aqueous solubilities of substrate and product can be used to predict if ISPR might be required. When a particular auxiliary phase is selected and the distribution coefficients of substrate and product are known, it is possible to estimate a priori if this auxiliary phase might be good enough and how much of it might be needed for an efficient (fed‐)batch biotransformation process. For biotransformation products of intermediate polarity (aqueous solubility of about 1–10 g/L) there seems to be a lack of extractants and adsorbents with the capacity to raise the product concentrations to commercially more interesting levels.