Premium
Quantitative Comparison of Lactose and Glucose Utilization in Bifidobacterium longum Cultures
Author(s) -
Kim TeakBum,
Song SuHan,
Kang SuCheol,
Oh DeokKun
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0257426
Subject(s) - lactose , bifidobacterium longum , food science , carbohydrate , actinomycetaceae , chemistry , biology , biochemistry , bifidobacterium , fermentation , lactobacillus
In batch cultures, Bifidobacterium longum SH2 has a higher final cell concentration and greater substrate consumption when grown on lactose versus glucose. Continuous cultures were used to compare lactose and glucose utilization by B . longum quantitatively. In the continuous culture, the estimated maintenance coefficients ( m ) were similar when on lactose and glucose; the maximum cell yield coefficient ( Y X/S max ) was higher on lactose; and the specific consumption rate of lactose ( q S ) was lower than that of glucose. Assuming that cell growth followed the Monod model, the maximum specific growth rates ( μ max ) and saturation constants ( K S ) in lactose and glucose media were determined using the Hanes‐Woolf plots. The respective values were 0.40 h − 1 and 78 mg/L for lactose and 0.46 h − 1 and 697 mg/L for glucose. The kinetic parameters of the continuous cultures showed that B . longum preferred lactose to glucose, although the specific consumption rate of glucose was higher than that of lactose.