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Potential of On‐Line CIMS for Bioprocess Monitoring
Author(s) -
Custer Thomas G.,
Wagner William P.,
Kato Shuji,
Bierbaum Veronica M.,
Fall Ray
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp025730k
Subject(s) - acetoin , chemistry , bioprocess , acetaldehyde , chromatography , isoprene , mass spectrometry , bioreactor , acetone , diacetyl , gas chromatography , reagent , butanol , volatile organic compound , ethanol , fermentation , organic chemistry , chemical engineering , engineering , copolymer , polymer
Chemical‐ionization mass spectrometry (CIMS) using flow reactors is an emerging method for on‐line monitoring of trace concentrations of organic compounds in the gas phase. In this study, a flow‐reactor CIMS instrument, employing the H 3 O + cation as the ionizing reagent, was used to simultaneously monitor several volatile metabolic products as they are released into the headspace during bacterial growth in a bioreactor. Production of acetaldehyde, ethanol, acetone, butanol, acetoin, diacetyl, and isoprene by Bacillus subtilis is reported. Ion signal intensities were related to solution‐phase concentrations using empirical calibrations and, in the case of isoprene, were compared with simultaneous gas chromatography measurements. Identification of volatile and semivolatile metabolites is discussed. Flow‐reactor CIMS techniques should be useful for bioprocess monitoring applications because of their ability to sensitively and simultaneously monitor many volatile metabolites on‐line.