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Development of a Phenomenological Modeling Approach for Prediction of Growth and Xanthan Gum Production Using Xanthomonas campestris
Author(s) -
Letisse Fabien,
Lindley Nic D.,
Roux Gilles
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0257168
Subject(s) - xanthan gum , xanthomonas campestris , ammonium nitrate , fermentation , biomass (ecology) , phosphate , ammonium phosphate , chemistry , yield (engineering) , nitrate , ammonium sulfate , ammonium , food science , biological system , thermodynamics , chromatography , biochemistry , rheology , agronomy , biology , organic chemistry , physics , fertilizer , gene
An unstructured kinetic model for xanthan production is described and fitted to experimental data obtained in a stirred batch reactor. The culture medium was composed of several nitrogen sources (soybean hydrolysates, ammonium and nitrate salts) consumed sequentially. The model proposed is able to describe this sequential consumption of nitrogen sources, the consumption of inorganic phosphate and carbon, the evolution of biomass, and production of xanthan. The parameter estimation has been performed by fitting the kinetic model in differential form to experimental data. Runs of the model for simulating xanthan gum production as a function of the initial concentration of inorganic phosphate have shown the positive effect of phosphate limitation on xanthan yield, though diminishing rates of production. The model was used to predict the kinetic parameters for a medium containing a 2‐fold lower initial phosphate concentration. When tested experimentally, the measured fermentation parameters were in close agreement with the predicted model values, demonstrating the validity of the model.