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Monitoring of Recombinant Protein Production Using Bioluminescence in a Semiautomated Fermentation Process
Author(s) -
Trezzani I.,
Nadri M.,
Dorel C.,
Lejeune P.,
Bellalou J.,
Lieto J.,
Hammouri H.,
Longin R.,
Dhurjati P.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp025714n
Subject(s) - bioluminescence , recombinant dna , fermentation , plasmid , bioprocess , bioreactor , intracellular , luciferase , biology , cell culture , biochemistry , chemistry , computational biology , gene , transfection , genetics , paleontology , botany
On‐line optimization of fermentation processes can be greatly aided by the availability of information on the physiological state of the cell. The goal of our “BioLux” research project was to design a recombinant cell capable of intracellular monitoring of product synthesis and to use it as part of an automated fermentation system. A recombinant plasmid was constructed containing an inducible promoter that controls the gene coding for a model protein and the genes necessary for bioluminescence. The cells were cultured in microfermenters equipped with an on‐line turbidity sensor and a specially designed on‐line light sensor capable of continuous measurement of bioluminescence. Initial studies were done under simple culture conditions, and a linear correlation between luminescence and protein production was obtained. Such specially designed recombinant bioluminescent cells can potentially be applied for model‐based inference of intracellular product formation, as well as for optimization and control of recombinant fermentation processes.