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Heat Inactivation of Mammalian Cell Cultures for Biowaste Kill System Design
Author(s) -
Gregoriades Niki,
Luzardo Melissa,
Lucquet Beatrice,
Ryll Thomas
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp025637w
Subject(s) - trypan blue , cell culture , viability assay , chemistry , hek 293 cells , cell , microbiology and biotechnology , biology , biochemistry , genetics
A biowaste kill system was implemented to treat biological waste generated from a clinical manufacturing and R&D antibody facility. To confirm that design parameters of this continuous decontamination system are sufficient to inactivate mammalian cell culture waste, bench‐scale experiments were conducted. The biowaste kill system heat inactivates mammalian cell cultures before they are piped to a neutralization tank and subsequently released to the sewage system. Heat inactivation of cells is accomplished by exposing cells to 80 °C for 1 min. Small‐scale heat inactivation studies were performed on CHO, 293‐HEK, and hybridoma cells. Cells at 1 × 10 6 cells/mL or 1 × 10 7 cells/mL were exposed to 37, 60, 70, or 80 °C for 0, 30, 60, and 120 s. Viability based on trypan blue exclusion method and ability to proliferate was assessed after exposure to heat. Data suggest that exposure of cells to 80 °C for 60 s is sufficient to inactivate these cultures before they are released to the sewage system.