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Efficient Lipid Delivery to Hybridoma Culture by Use of Cyclodextrin in a Novel Granulated Dry‐Form Medium Technology
Author(s) -
Walowitz Jennifer L.,
Fike Richard M.,
Jayme David W.
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp025590e
Subject(s) - chemistry , granulation , cyclodextrin , food science , chromatography , biopharmaceutical , filtration (mathematics) , biochemistry , microbiology and biotechnology , materials science , biology , mathematics , statistics , composite material
Lipids are critical nutrients for high density eukaryotic cell cultivation, but inclusion of lipid components into dry‐form media has been technically challenging. Lipid supplements are usually supplied for separate addition after powder reconstitution and filtration, which increases manipulation and risk in a biopharmaceutical manufacturing facility. Advanced Granulation Technology (AGT) is a novel dry‐form media format designed for large‐scale biopharmaceutical manufacture. All components of a complex formulation are homogeneously incorporated into a single granulated medium that simply requires water addition to yield a complete reconstituted 1X medium. We investigated whether cyclodextrin technology could be combined with the AGT process to deliver usable lipid in a dry medium format. The test lipids were cholesterol and several fatty acids supplied either as an aseptic supplement to liquid media or as part of a complete AGT formulation. The test system utilized a cholesterol auxotroph, NS0, and a protein‐free basal formulation (CD Hybridoma Medium), with or without supplemental lipid. Post‐filtration recovery of cholesterol from AGT‐processed materials compared favorably with media supplemented with liquid lipid concentrates. Cell growth and viability and expression of recombinant protein were equivalent in all test media.