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Magnetophoretic Cell Sorting Is a Function of Antibody Binding Capacity
Author(s) -
McCloskey Kara E.,
Moore Lee R.,
Hoyos Mauricio,
Rodriguez Alex,
Chalmers Jeffrey J.,
Zborowski Maciej
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp020285e
Subject(s) - magnetic separation , cell sorting , elution , immunomagnetic separation , cell , sorting , flow cytometry , chemistry , chromatography , materials science , microbiology and biotechnology , biology , computer science , biochemistry , metallurgy , programming language
Antibody binding capacity (ABC) is a term representing a cellapos;s ability to bind antibodies, correlating with the number of specific cellular antigens expressed on that cell. ABC allows magnetically conjugated antibodies to bind to the targeted cells, imparting a magnetophoretic mobility on each targeted cell. This enables sorting based on differences in the cell magnetophoretic mobility and, potentially, a magnetic separation based on the differences in the cell ABC values. A cellapos;s ABC value is a particularly important factor in continuous magnetic cell separation. This work investigates the relationship between ABC and magnetic cell separation efficiency by injection of a suspension of immunomagnetically labeled quantum simply cellular calibration microbeads of known ABC values into fluid flowing through a quadrupole magnetic sorter. The elution profiles of the outlet streams were evaluated using UV detectors. Optimal separation flow rate was shown to correlate with the ABC of these microbeads. Comparing experimental and theoretical results, the theory correctly predicted maximum separation flow rates but overestimated the separation fractional recoveries.